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Flow through Technology

Flow-through Hybridization technology is developed based on conventional hybridization. The uniqueness of "Flow-through" hybridization is by directing the amplicons toward the DNA probes to form duplexes, the "Flow-through" hybridization is changing from traditional passive to active channeling process allowing the recombination reactions to complete in seconds.

DNA hybridization assays are known to have excellent signal-to-noise ratio. It enables unambiguous detection of multiple pathogens/disease genotypes in one single reaction. However, a typical DNA hybridization step takes hours to complete.

Flow-Through Hybridization Technology is developed upon the DNA hybridization method. It simplifies the workflow and shortens the assay time but gives results with equally excellent signal-to-noise ratios.

Flow-through Hybridization Platform

A flow-though hybridization platform is the perfect technological tool for multiplexing. The platform is based on Flow-through hybridization technology for extremely efficient detection of multiple parameters. The Flow-through assays deliver users with excellent sensitivity and specificity in a highly efficient and economical setting which can maximize the lab productivity and service quality. A fully automatic platform for several molecular diagnostics applications and identification of possible pathogenic bio-targets in humans.

Safety

The fully automated platform is reducing and limiting the human activity in the laboratory procedure, and hence increasing safety for lab technicians. Furthermore, the platform has been developed to perform molecular diagnostics applications (ex. detection of post-PCR products), where safety for lab technicians using the traditional methodologies (ex. gel electrophoresis) is critical, and hence it avoids the contact of human personnel with dangerous products (ex. Ethidium Bromide for gel electrophoresis).

Accuracy and precision

The platform has been conceived in order to guarantee the maximum standards of quality in terms of capability to reach the desired temperatures with very low CVs, high rates of sensibility and specificity as well as the possibility to reproduce the same results over the time without any difference. These quality standards in terms of accuracy and precision enable the platform to be a perfect tool for molecular diagnostics applications.

Reliance and efficiency

The platform is conceived with top level technologies and high reliable components, in order to guarantee the maximum reliability and stability in terms of step processing over the time.

The platform is also extremely efficient: Flow-Through hybridization technology overcomes the diffusion limitation and significantly increases the kinetics of hybridization process.

Automation and Easyness

The full automation of the platform guarantees high processes reproducibility, fastness, sensible reduction in human mistakes and cross-contamination, as well as reduced human intervention. In fact the platform is really easy in the operations, and after starting the procedures the laboratory technician will only need to come back at the end to recover the results.

Specs

  • Temperature uniformity: in the reaction chamber ± 0.25 °C
  • Temperature range: 25-70 °C treached by the reaction chamber in general.
  • Reagent positions: 8 at room Temperature + 1 to incubate at 40-45 °C
  • Pipetteand tips: the robotic arm includes a pipette able to dispense 50-1200 µl reagents. Tips are taken from the tips tray and after the use are discarded in a specific bag for used tips.
  • Reaction chamber: The reaction chamber is characterized by 15 positions.
  • Samples: A sample block is able to allow PCR tubes
  • Detection: One CCD camera is on the top of the robotic arm for the acquisition step. Images acquired are installed within the instrument and one software for image analysis and interpretation of the results provide the final reports.
  • Size: The dimension of the instruments are 450x450x600 mm (LxDxH)
  • Productivity: 1h for a full run of maximum 15 samples per run (minimum 1 sample per run)